On leishmanial antigen detection in tissue sections of Montenegro's reaction

BHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry

Background: Respiratory tract infections caused by some viruses with cattle origin have been demonstrated in sheep and goats. OBJECTIVES: The main goal of this study was to determine Bovine Herpes virus type 1 BHV1antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic sheep, using immunohistochemistry (IHC) staining method. METHODS: For this purpose, the lungs of 4079 sheep, which...

The Use of indirect Immunoperoxidase Assay in Diagnosis of Type A (H9N2) Avian Influenza Virus Antigen on Frozen Tissue Sections

Denovirus Antigen Detection in Paraffinized Lung Sections of Pneumonic Goat Lungs Using Immunohistochemistry

BACKGROUNDS: Diseases affecting the respiratory tract of sheep and goats are one of the most important factors which limit production of these species on a world-wide basis. OBJECTIVES: The main goal of this study was to determine Adenovirus (AdV) antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic goats, using immunohistochemistry (IHC) staining method. METHODS: For this purpo...

Detection of Bromodeoxyuridine in Paraffin- embedded Tissue Sections Using Microwave Antigen Retrieval is Dependent on the Mode of Tissue Fixation

bstract A simple routine microwave antigen retrieval procedure allows the sensitive detection of incorporated BrdU in pulse labeled cells. Of the two fixatives tested, Carnoy’s offers superior nuclear morphology, but with a sacrifice of immunostaining intensity. For investigations where animals are sacrificed within several hours after pulse labeling, Carnoy‘s fixative may prove adequate as a g...

Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction.

The use of in situ techniques to detect DNA and RNA sequences has proven to be an invaluable technique with paraffin-embedded tissue. Advances in non-radioactive detection systems have further made these procedures shorter and safer. We report the detection of Trypanosoma cruzi, the causative agent of Chagas disease, via indirect and direct in situ polymerace chain reaction within paraffin-embe...